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Description
Cardiac Troponins I (cTnI) His Tag Protein, HumanProduct Specification Species Human Synonyms cardiac troponin I,Human Cardiac Troponin I; cardiac troponin I,Human Cardiac Troponin I Accession P19429 Amino Acid Sequence MHHHHHHDDDDKADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES. Expression System E. coli Molecular Weight 28 kDa Purity 95%,
Product Specification
| Species | Human |
| Synonyms | cardiac troponin-I,Human Cardiac Troponin I;cardiac troponin-I,Human Cardiac Troponin I |
| Accession | P19429 |
| Amino Acid Sequence | MHHHHHHDDDDKADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES. |
| Expression System | E.coli |
| Molecular Weight | 28 kDa |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, 2M Urea, pH8.0 |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Cardiac Troponins I (cTnI), the Chinese name cardiac troponin I subunit, and cardiac troponin T subunit and cardiac troponin C subunit constitute cardiac troponin complex. Cardiac troponin complex is a calcium regulatory protein, which is used to regulate the contractile function of skeletal muscle and myocardium. When the concentration of intracellular Ca2+ is low, cTnI subunit can inhibit the interaction between troponin and troponin, and then inhibit the formation of myofibrinin. At present, cTnI is only found in cardiomyocytes and is released into the bloodstream after myocardial injury. Therefore, cTnI has high myocardial specificity and sensitivity, and is an important biomarker in the early diagnosis of acute myocardial infarction.
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