SKU: 33446917719

UA-Glo® cAMP Reagent

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Description

UA-Glo® cAMP ReagentProduct Specification Stability & Storage Dry ice transportation. Store protected from light at 80C. Upon initial use, prepare as UA Glo cAMP reagent stock solution, then aliquot as needed and store protected from light at 80C, avoiding repeated freeze thaw cycles. Background The UA Glo cAMP reagent is suitable for measuring changes in cAMP concentration in live cells using the Promega GloSensor cAMP assay system, with detection performance comparable

Product Specification


Stability & Storage

Dry ice transportation. Store protected from light at -80°C. Upon initial use, prepare as UA-Glo® cAMP reagent stock solution, then aliquot as needed and store protected from light at -80°C, avoiding repeated freeze-thaw cycles.

Background

The UA-Glo® cAMP reagent is suitable for measuring changes in cAMP concentration in live cells using the Promega GloSensor™ cAMP assay system, with detection performance comparable to that of the Promega GloSensor™ cAMP Reagent (Cat E1290, E1291).

Components

The components and specifications of UA-Glo® cAMP reagent are as follows. The detectable number of wells in 96-/384-well plates is calculated based on the standard experimental conditions where 100/20 μL of balanced medium is added per well in 96-/384-well plates, respectively.

cAMP Reagent

HEPES Buffer (10mM, pH7.5)

Detectable 96-Well Plate Wells

Detectable 384-Well Plate Wells

25 mg

1 mL

2,000

250 mg

10 mL

20,000


Protocol

According to the Promega GloSensor™ cAMP assay system manual (TM076), design and perform relevant experiments. Below are the experimental steps for cAMP detection in adherent cells using UA-Glo® cAMP reagent.

1)Prepare UA-Glo® cAMP reagent stock solution: Add 25mg UA-Glo® cAMP reagent (L7101) to 817μL of 10mM HEPES buffer (pH7.5), or add 250mg UA-Glo® cAMP reagent (L7102) to 8.17mL HEPES buffer (pH7.5). Mix thoroughly. Aliquot as needed and store protected from light at -80, avoiding repeated freeze-thaw cycles. This cAMP reagent stock solution can be diluted 50x as described in step 3 to prepare equilibration medium, or the concentration of cAMP reagent in the equilibration medium can be optimized for maximum signal-to-background difference.

2)Prepare equilibration medium aseptically as needed:

88% CO2-independent medium (e.g., Invitrogen Cat.#18045 )

10% fetal bovine serum

2% UA-Glo® cAMP reagent stock solution

3)Remove the 96-well experimental cell plate (e.g., cells expressing Promega GloSensor™ or transiently transfected cells), carefully aspirate the medium from the wells, and gently add 100μL of the equilibration medium prepared in step 2. Incubate at room temperature (22-25) for 2hr. The incubation time and temperature can be optimized based on when background signals stabilize. Ensure consistent temperature across the plate during detection.

4)Add test compounds and appropriate controls according to the experimental design. For example, use 10μM forskolin as a positive control and the solvent vehicle as a negative control.

5)Measure luminescence signals using a luminometer or multifunctional plate reader 15-30min after compound addition. Refer to section 3.A of the Promega GloSensor™ cAMP assay system manual (TM076) for specific timing or optimize based on the experiment.

Guidelines

1) Different batches are not recommended for mixed use 2) It is not recommended to change the dosage of detection reagents without strict validation 3) For research use only
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SKU: 33446917719

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Mama Seeks Magic
Waukegan, US
★★★★★ 5
Moisturizing, effective, pleasantly scented sunscreen
Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1), Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1)
This is the go-to sunscreen our whole family has been using for years. It feels moisturizing (but not greasy) and is easy to rub in to avoid any kind of chalky, white residue. The scent is subtle and pleasant, not the strong pronounced sunscreen smell that a lot of others have. Best of all, it is effective! The bottle received is different than the one pictured on Amazon, but we’re not buying it for the bottle, so that’s okay. :) Would recommend.
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Reviewed in the United States on July 27, 2022
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Johanna Jimenez
Massapequa, US
★★★★★ 5
Wonderful
Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1)
I love it! My daughters have very delicate skin and this product works wonders.
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Reviewed in the United States on October 27, 2025
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Drug Waitress, RN
Dallas, US
★★★★★ 5
I hate sunscreens, but...
Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1)
I hate sunscreen in general--for it's texture and smell. But, being the palest family on Earth makes it a daily necessity. I was pleasantly surprised to actually like the smell of this product! It's a light fruity fragrance. It has a smooth texture like lotion and glided on my skin without being too thick or greasy and no white marks. A little goes a long way too! I could hardly believe I was putting on sunscreen and not regular body lotion. My kids had no issue with it either! They let me put this stuff on their little faces without complaint since it goes on so smoothly. Most importantly, this product did it's job and we were sunburn free. The tube is small--4 ounces--but I don't care because it's so worth having something that goes on easy and doesn't make us greasy and stinky. I bought several tubes--one for each member of the family. *If this review was helpful, please click 'yes' below. Thanks!*
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Reviewed in the United States on June 14, 2018
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Jill W
Belleville, US
★★★★★ 4
It’s ok
Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1)
Doesn’t smell like anything, especially not fruit. Seems to work well- it’s what we use on our faces. I started using this all over on my daughter because their spray version is not good.
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Reviewed in the United States on April 24, 2019
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Cosmos1
Pawtucket, US
★★★★★ 5
Overall this is a very good product
Style: Kids SPF 50, Size: 3 Fl Oz (Pack of 1)
I bought this because every sunblock I own eventually gets in my eyes and burns the living daylights out of them. I prefer to use something that isn't laden with chemicals, is cruelty free and reef safe so this looked like it would fit the bill and indeed, it does. It is a true "no tears" formula which I very much appreciate. As with so many zinc based sunblocks there's still a slight "Kabuki mask" effect but it isn't as bad as some of the other zinc products. I wouldn't go so far as to say it's easy to spread because it doesn't have a lot of "slip", doesn't glide like the non-zinc products. You do have to work with it for awhile to get rid of the decidedly chalky consistency but once you get going it seems to be okay. I think somebody else mentioned using a moisturizer before you put it on which seems like good idea even though this is supposed to be moisturizing. Would I purchase this again? I'm pretty sure I will because the "no tears" aspect of the product is important to me. I'll probably use it with a light carrier oil on my face first, let that oil sink in and then put this on top. I'll keep you posted on how that works out. Thank you, Alba Botanica, for being cruelty free and reef safe!
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Reviewed in the United States on August 22, 2020

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