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Description
Biotin Rat Anti-Mouse Perforin Antibody (S-R644)Product Specification Host Rat Antigen Perforin Synonyms Perforin 1; P1; Cytolysin; Lymphocyte pore forming protein; Pfp; Prf1 Location Secreted, Cell membrane Accession P10820 Clone Number S R644 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample BALB c mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Biotin Physical Appearance Liquid Storage Buffer PBS pH7. 4, 0. 03% Proclin 300 Stability
Product Specification
| Host | Rat |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1 |
| Location | Secreted, Cell membrane |
| Accession | P10820 |
| Clone Number | S-R644 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.
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Exchange/Return Notes
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