SKU: 18496151630

5x Kinase Buffer A

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Description

5x Kinase Buffer AProduct Specification Physical Appearance Liquid Stability & Storage For long term storage, keep 5X buffer at 20C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4C for 1 2 weeks. Background Kise Buffer can be used to assay protein kise activity. Components 200 mM Tris HCl, pH 7. 4, 100 mM MgCl2, and 0. 5 mg mL BSA. Add fresh DTT prior to use to a fil concentration of 250 M. Protocol 1. Thaw 5X buffer at 24 30C,

Product Specification


Physical Appearance Liquid
Stability & Storage

For long term storage, keep 5X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks.

Background

Kise Buffer can be used to assay protein kise activity.

Components

200 mM Tris-HCl, pH 7.4, 100 mM MgCl2, and 0.5 mg/mL BSA. Add fresh DTT prior to use to a fil concentration of 250 μM.

Protocol

1. Thaw 5X buffer at 24-30°C, mixing end-over-end.

2. Dilute the 5X buffer to the desired concentration using ddH2O.

Guidelines

For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable

performance, avoid repeated handling and multiple freeze/thaw cycles.

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SKU: 18496151630

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